Optimizing functional imaging protocols for assessing the outcome of fetal cell transplantation in Parkinson's disease
© Politis; licensee BioMed Central Ltd. 2011
Received: 6 April 2011
Accepted: 10 May 2011
Published: 10 May 2011
Clinical trials aiming to assess the safety and efficacy of fetal cell transplantation in Parkinson's disease rely on the hypothesis that the grafted tissue will survive and grow, restore striatal dopaminergic neurotransmission, improve the connectivity between striatum, thalamus and cortex and, thereby, produce long-lasting clinical improvement while avoiding the development of adverse effects. Although transplantation of human fetal ventral mesencephalic tissue has been reported as one of the most effective reparative therapies in Parkinson's disease patients to date, different studies have shown inconsistent results causing a paucity of new trials over the last decade. However, during this period, functional imaging alongside other scientific developments from clinical observations and animal work has significantly aided in understanding the mechanisms responsible for the success or failure of grafting human fetal tissue. Recent advances in functional imaging including both positron emission tomography and functional magnetic resonance imaging could be proven useful in vivo tools for the development and assessment of new clinically competitive trials. In this commentary we discuss how an optimized functional imaging protocol could assist new clinical trials using fetal cell transplantation in Parkinson's disease.
In previous trials, functional imaging using mainly positron emission tomography (PET) has provided objective in vivo evidence that human dopamine (DA) -rich fetal ventral mesencephalic (VM) tissue implanted in the striatum of Parkinson's disease (PD) patients can survive, grow, release DA, normalize brain metabolism and restore striatal-cortical connections, clinically corresponding to significant symptomatic relief in some cases [1–3].
Positron emission tomography techniques
Aromatic amino acid decarboxylase (AADC)
Provides measures of AADC activity and allows an indirect measure of DA storage within the DA terminals.
Post-synaptic DA D2 receptors
Is a subject to competitive displacement by endogenous DA. Acute administration of substances such as amphetamine, methylphenidate, or L-DOPA, which are known to increase the levels of extracellular DA result in a reduction of 11C-raclopride binding.
H2 15O PET
Regional cerebral blood flow (rCBF)
Brain activation and regional cerebral metabolism.
Dopamine transporter (DAT)
Marker of presynaptic DA terminals integrity and DAT availability
Marker of presynaptic DA terminals integrity and DAT availability
Marker of presynaptic DA terminals integrity and DAT availability
Translocator protein (TSPO)
Is an 18 kDa protein of the outer mitochondrial membrane that is upregulated with activation of microglia
5-HT transporter (SERT)
Marker of presynaptic 5-HT terminals integrity and SERT availability
11C-dihydrotetrabenazine (DTBZ) PET
Vesicular monoamine transporter (VMAT2)
Marker of presynaptic monoaminergic terminals integrity
Advances in imaging techniques including both PET and magnetic resonance imaging (MRI) could further assist in the development and monitoring of new clinically competitive trials.
Despite the lack of new clinical trials since the beginning of the previous decade, retrospective analysis of data, studies in animal models and long-term clinical and imaging observations in the earlier transplanted PD patients have allowed us to understand a number of ways that functional imaging could help in understanding and monitoring outcomes of fetal cell transplantation trials in PD.
There are now up to 16 years of post-transplantation 18F-DOPA PET follow-up data showing graft viability and continuous clinical benefit in several transplanted PD patients with fetal VM tissue [11, 13–15]. As the degree of motor impairment has been shown to correlate with 18F-DOPA uptake in the striata of PD patients , clinical outcomes following fetal cell transplantation could be associated with the number of viable DA cells innervating the striatum as assessed by 18F-DOPA PET. This notion could explain the differences in the outcomes between open-label trials reporting an average of 50 to 85% increase in 18F-DOPA uptake associated with good clinical improvement of PD motor symptoms and reductions in medication requirements [4–8], and the two double-blind sham-surgery controlled clinical trials reporting an average of 20 to 40% increases in 18F-DOPA uptake associated with poor clinical outcomes during the first periods post-transplantation [9, 10].
18F-DOPA PET can also help facilitate patient selection and screening as patients with baseline reductions in 18F-DOPA uptake extending to the ventral part of the striatum and patients with reductions in 18F-DOPA uptake consistent with atypical or secondary Parkinsonism should be excluded from these trials [13, 17]. This knowledge, which derived from post-hoc analysis, raises the possibility of a time window for optimal transplantation outcomes as preoperative preservation of DA innervation in ventral striatal areas seems to be predictive of a better outcome.
During past years, a small number of studies have been undertaken in order to demonstrate imaging of DA transporters (DAT) in the grafted striatum. Imaging of DAT with PET or single photon emission computed tomography (SPECT) could be used as an alternative technique to 18F-DOPA PET. However, in line with the inconsistent results observed in post-mortem studies with regard to DAT expression in the grafted neurons [18, 19], 76Br-FE-CBT PET failed to visualize DAT in grafted striata enhancing 18F-DOPA , whereas 123I-IPT  and 123I-FP-CIT  SPECT showed robust DAT availability and compatible uptake to 18F-DOPA.
Up to 10 years post-transplantation follow-up data using 11C-raclopride PET have shown several cases where graft-derived DA cells were able to restore the release of endogenous DA in the striatum of PD patients [11, 14, 17]. The results of these studies suggested that it is very likely that the efficient restoration of DA release in large parts of the grafted striatum underlies patients' clinical improvement of motor symptoms. Moreover, from PET studies using H2 15O we learned that despite an early stabilization of DA reinnervation in the striatum, graft function and integration continue and symptomatic relief may also require the functional reafferentation of striato-thalamo-cortical circuitries in the host brain .
Immunosuppression could be another factor related to the outcomes of clinical trials, although the role of neuroinflammation in influencing the outcome of transplantation procedures in PD is not known. There is evidence showing that long-term immunosuppresion can be withdrawn without interfering with graft survival or the motor recovery induced by transplantation . Additional evidence indicated that the occurrence of graft-induced dyskinesias (GIDs), one of the most disabling side effects of cell therapy in PD, could be induced by inflammatory and immune responses around the graft. In one study GIDs developed after discontinuation of immunosuppressive therapy, with signs of an inflammatory reaction around the grafts in autopsied cases . Although imaging studies have never been employed, PET imaging can be used to explore the possible role of host inflammatory reaction in relation to fetal cell transplantation outcomes in PD using markers of microglial activation such as 11C-PK11195 PET or any of the other recently developed translocator protein (TSPO) PET ligands.
GIDs are a severe adverse effect of fetal cell transplantation in PD hindering the further development of cell transplantation trials [9, 10, 14, 15, 22–25]. Notwithstanding the several theories proposed , recent data imply that the development of GIDs could be related to the composition of grafted tissue , as human fetal VM tissue contains a varied proportion of non-DA neurons , including serotonin (5-HT) neurons. 11C-DASB PET and a clinical trial with an agent dampening transmitter release from 5-HT neurons was used to show a causal relationship between mishandling of DA release from the graft-derived 5-HT hyperinnervation in the striatum of PD patients and the occurrence of GIDs [14, 15]. Furthermore, it was suggested that the high ratios between 5-HT and DA neurons and between 5-HT transporters (SERT) and DAT could be the driving factor for the development of GIDs [14, 15, 25]. Therefore, these results suggest that achieving normal striatal 5-HT/DA and SERT/DAT ratios following transplantation of fetal tissue or stem cells should be necessary to avoid the development of GIDs.
Recent advances in MRI techniques allow us to examine the brain in a way that was not previously possible. The examination of functional connectivity between brain regions is now possible using resting-state functional MRI (fMRI). These resting state networks (RSNs) are believed to reflect functional communication between brain regions and could be very informative in providing insights of large-scale neuronal communication during restorative therapies such as fetal cell transplantation in PD [27–29]. FMRI paradigms with motor execution (ME) tasks are now widely employed to understand brain activation in the corresponding regions. Diffusion Tensor Imaging (DTI) offers a method to assess white matter (WM) structural connectivity and thereby, alterations underlying neurodegenerative diseases such as PD and the potential effects of restorative therapies [28, 30].
A new European Commission-funded multicenter trial under the name Transeuro was recently launched for a new round of fetal cell transplantation trials http://www.transeuro.org.uk. Functional imaging protocols for this or any other future trials employing fetal or stem cells will undoubtedly benefit from the lessons learned from over two decades of research.
Examples of imaging techniques used in the past and that can be used in the future
DA-rich graft survival and growth
DA release from the graft
11C-raclopride with challenge
11C-raclopride with challenge
Graft-derived 5-HT innervation and growth
11C-DASB PET/18F-DOPA PET ratio
11C-DASB PET/123I-FP-CIT SPECT ratio
Inflammatory and immune responses around the graft
Brain activation during movement
H2 15O PET with motor execution tasks
fMRI with motor execution tasks
Functional connectivity between brain regions
White matter structural connectivity
Diffusion Tensor Imaging
Although functional imaging cannot currently be used as a primary endpoint in clinical transplantation trials, if used appropriately, it can provide researchers with an additional valuable in vivo tool alongside clinical observations. It is worth noting, however, that previous knowledge has shown that in order to efficiently monitor cell replacement therapies in PD with functional imaging, long period follow-up assessments are needed and conclusions cannot be ultimately achieved with short follow-up periods.
Diffusion Tensor Imaging
functional magnetic resonance imaging
positron emission tomography
resting state networks
single photon emission computed tomography
vesicular monoamine transporter 2
- Lindvall O, Hagell P: Clinical observations after neural transplantation in Parkinson's disease. Prog Brain Res. 2000, 127: 299-320.View ArticlePubMedGoogle Scholar
- Brooks DJ: Positron emission tomography imaging of transplant function. NeuroRx. 2004, 1: 482-491. 10.1602/neurorx.1.4.482.View ArticlePubMedPubMed CentralGoogle Scholar
- Lindvall O, Björklund A: Cell therapy in Parkinson's disease. NeuroRx. 2004, 1: 382-393. 10.1602/neurorx.1.4.382.View ArticlePubMedPubMed CentralGoogle Scholar
- Freeman TB, Olanow CW, Hauser RA, Nauert GM, Smith DA, Borlongan CV, Sanberg PR, Holt DA, Kordower JH, Vingerhoets FJ, Snow BJ, Calne D, Gauger LL: Bilateral fetal nigral transplantation into the postcommissural putamen in Parkinson's disease. Ann Neurol. 1995, 38: 379-388. 10.1002/ana.410380307.View ArticlePubMedGoogle Scholar
- Remy P, Samson Y, Hantraye P, Fontaine A, Defer G, Mangin JF, Fénelon G, Gény C, Ricolfi F, Frouin V, N'Guyen JP, Jeny R, Degos JD, Peschanski M, Cesaro P: Clinical correlates of [18F]fluorodopa uptake in five grafted parkinsonian patients. Ann Neurol. 1995, 38: 580-588. 10.1002/ana.410380406.View ArticlePubMedGoogle Scholar
- Wenning GK, Odin P, Morrish P, Rehncrona S, Widner H, Brundin P, Rothwell JC, Brown R, Gustavii B, Hagell P, Jahanshahi M, Sawle G, Björklund A, Brooks DJ, Marsden CD, Quinn NP, Lindvall O: Short- and long-term survival and function of unilateral intrastriatal dopaminergic grafts in Parkinson's disease. Ann Neurol. 1997, 42: 95-107. 10.1002/ana.410420115.View ArticlePubMedGoogle Scholar
- Hagell P, Schrag A, Piccini P, Jahanshahi M, Brown R, Rehncrona S, Widner H, Brundin P, Rothwell JC, Odin P, Wenning GK, Morrish P, Gustavii B, Björklund A, Brooks DJ, Marsden CD, Quinn NP, Lindvall O: Sequential bilateral transplantation in Parkinson's disease: effects of the second graft. Brain. 1999, 122: 1121-1132. 10.1093/brain/122.6.1121.View ArticlePubMedGoogle Scholar
- Brundin P, Pogarell O, Hagell P, Piccini P, Widner H, Schrag A, Kupsch A, Crabb L, Odin P, Gustavii B, Björklund A, Brooks DJ, Marsden CD, Oertel WH, Quinn NP, Rehncrona S, Lindvall O: Bilateral caudate and putamen grafts of embryonic mesencephalic tissue treated with lazaroids in Parkinson's disease. Brain. 2000, 123: 1380-1390. 10.1093/brain/123.7.1380.View ArticlePubMedGoogle Scholar
- Freed CR, Greene PE, Breeze RE, Tsai WY, DuMouchel W, Kao R, Dillon S, Winfield H, Culver S, Trojanowski JQ, Eidelberg D, Fahn S: Transplantation of embryonic dopamine neurons for severe Parkinson's disease. N Engl J Med. 2001, 344: 710-719. 10.1056/NEJM200103083441002.View ArticlePubMedGoogle Scholar
- Olanow CW, Goetz CG, Kordower JH, Stoessl AJ, Sossi V, Brin MF, Shannon KM, Nauert GM, Perl DP, Godbold J, Freeman TB: A double-blind controlled trial of bilateral fetal nigral transplantation in Parkinson's disease. Ann Neurol. 2003, 54: 403-414. 10.1002/ana.10720.View ArticlePubMedGoogle Scholar
- Piccini P, Brooks DJ, Björklund A, Gunn RN, Grasby PM, Rimoldi O, Brundin P, Hagell P, Rehncrona S, Widner H, Lindvall O: Dopamine release from nigral transplants visualized in vivo in a Parkinson's patient. Nat Neurosci. 1999, 2: 1137-1140. 10.1038/16060.View ArticlePubMedGoogle Scholar
- Piccini P, Lindvall O, Björklund A, Brundin P, Hagell P, Ceravolo R, Oertel W, Quinn N, Samuel M, Rehncrona S, Widner H, Brooks DJ: Delayed recovery of movement-related cortical function in Parkinson's disease after striatal dopaminergic grafts. Ann Neurol. 2000, 48: 689-695. 10.1002/1531-8249(200011)48:5<689::AID-ANA1>3.0.CO;2-N.View ArticlePubMedGoogle Scholar
- Ma Y, Tang C, Chaly T, Greene P, Breeze R, Fahn S, Freed C, Dhawan V, Eidelberg D: Dopamine cell implantation in Parkinson's disease: long-term clinical and (18)F-FDOPA PET outcomes. J Nucl Med. 2010, 51: 7-15. 10.2967/jnumed.109.066811.View ArticlePubMedGoogle Scholar
- Politis M, Wu K, Loane C, Quinn NP, Brooks DJ, Rehncrona S, Bjorklund A, Lindvall O, Piccini P: Serotonergic neurons mediate dyskinesia side effects in Parkinson's patients with neural transplants. Sci Transl Med. 2010, 2: 38ra46-10.1126/scitranslmed.3000976.View ArticlePubMedGoogle Scholar
- Politis M, Oertel WH, Wu K, Quinn NP, Pogarell O, Brooks DJ, Bjorklund A, Lindvall O, Piccini P: Graft-induced dyskinesias in Parkinson's disease: high striatal serotonin/dopamine transporter ratio. Mov Disord.
- Morrish PK, Sawle GV, Brooks DJ: An [18F]dopa-PET and clinical study of the rate of progression in Parkinson's disease. Brain. 1996, 119: 585-591. 10.1093/brain/119.2.585.View ArticlePubMedGoogle Scholar
- Piccini P, Pavese N, Hagell P, Reimer J, Björklund A, Oertel WH, Quinn NP, Brooks DJ, Lindvall O: Factors affecting the clinical outcome after neural transplantation in Parkinson's disease. Brain. 2005, 128: 2977-2986. 10.1093/brain/awh649.View ArticlePubMedGoogle Scholar
- Kordower JH, Rosenstein JM, Collier TJ, Burke MA, Chen EY, Li JM, Martel L, Levey AE, Mufson EJ, Freeman TB, Olanow CW: Functional fetal nigral grafts in a patient with Parkinson's disease: chemoanatomic, ultrastructural, and metabolic studies. J Comp Neurol. 1996, 370: 203-230. 10.1002/(SICI)1096-9861(19960624)370:2<203::AID-CNE6>3.0.CO;2-6.View ArticlePubMedGoogle Scholar
- Kordower JH, Chu Y, Hauser RA, Freeman TB, Olanow CW: Lewy body-like pathology in long-term embryonic nigral transplants in Parkinson's disease. Nat Med. 2008, 14: 504-506. 10.1038/nm1747.View ArticlePubMedGoogle Scholar
- Cochen V, Ribeiro MJ, Nguyen JP, Gurruchaga JM, Villafane G, Loc'h C, Defer G, Samson Y, Peschanski M, Hantraye P, Cesaro P, Remy P: Transplantation in Parkinson's disease: PET changes correlate with the amount of grafted tissue. Mov Disord. 2003, 18: 928-932. 10.1002/mds.10463.View ArticlePubMedGoogle Scholar
- Pogarell O, Koch W, Gildehaus FJ, Kupsch A, Lindvall O, Oertel WH, Tatsch K: Long-term assessment of striatal dopamine transporters in Parkinsonian patients with intrastriatal embryonic mesencephalic grafts. Eur J Nucl Med Mol Imaging. 2006, 33: 407-411. 10.1007/s00259-005-0032-z.View ArticlePubMedGoogle Scholar
- Hagell P, Piccini P, Björklund A, Brundin P, Rehncrona S, Widner H, Crabb L, Pavese N, Oertel WH, Quinn N, Brooks DJ, Lindvall O: Dyskinesias following neural transplantation in Parkinson's disease. Nat Neurosci. 2002, 5: 627-628.PubMedGoogle Scholar
- Ma Y, Feigin A, Dhawan V, Fukuda M, Shi Q, Greene P, Breeze R, Fahn S, Freed C, Eidelberg D: Dyskinesia after fetal cell transplantation for Parkinsonism: a PET study. Ann Neurol. 2002, 52: 628-634. 10.1002/ana.10359.View ArticlePubMedGoogle Scholar
- Olanow CW, Gracies JM, Goetz CG, Stoessl AJ, Freeman T, Kordower JH, Godbold J, Obeso JA: Clinical pattern and risk factors for dyskinesias following fetal nigral transplantation in Parkinson's disease: a double blind video-based analysis. Mov Disord. 2009, 24: 336-343. 10.1002/mds.22208.View ArticlePubMedGoogle Scholar
- Politis M: Dyskinesias after neural transplantation in Parkinson's disease: what do we know and what is next?. BMC Med. 2010, 8: 80.View ArticlePubMedPubMed CentralGoogle Scholar
- Isacson O, Bjorklund LM, Schumacher JM: Toward full restoration of synaptic and terminal function of the dopaminergic system in Parkinson's disease by stem cells. Ann Neurol. 2003, 53 (Suppl 3): 135-148.View ArticleGoogle Scholar
- Greicius M: Resting-state functional connectivity in neuropsychiatric disorders. Curr Opin Neurol. 2008, 21: 424-430.View ArticlePubMedGoogle Scholar
- Guye M, Bartolomei F, Ranjeva JP: Imaging structural and functional connectivity: towards a unified definition of human brain organization?. Curr Opin Neurol. 2008, 21: 393-403.View ArticlePubMedGoogle Scholar
- Bullmore E, Sporns O: Complex brain networks: graph theoretical analysis of structural and functional systems. Nat Rev Neurosci. 2009, 10: 186-198. 10.1038/nrn2575.View ArticlePubMedGoogle Scholar
- Ciccarelli O, Catani M, Johansen-Berg H, Clark C, Thompson A: Diffusion-based tractography in neurological disorders: concepts, applications, and future developments. Lancet Neurol. 2008, 7: 715-727. 10.1016/S1474-4422(08)70163-7.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1741-7015/9/50/prepub
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