Pinocembrin protected mitochondrial function and inhibited nuclear apoptosis. (A) Fluorescent images of mitochondrial function and nuclear changes in the same field. The mitochondria were monitored using the fluorescent dyes, Rh123 and MitoSOX Red, in living cells. Fluorescent images and intensities were acquired and analyzed by a Cellomics ArrayScan high-content screening (HCS) Reader combined with the Cell Health Profiling BioApplication Guide provided with the BioApplication software. (B-D) Values of Mean_AvgInten of Rh123, MitoSOX Red and Hoechst 33342 collected from the respective fluorescent channels using the same optical field. Data are expressed as mean ± SEM, n = 6, **P < 0.01, ***P < 0.001 vs control, #
P < 0.05, ###
P < 0.001 vs copper.