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Table 1 Primary antibodies and secondary antibodies used in this study

From: Pinocembrin protects against β-amyloid-induced toxicity in neurons through inhibiting receptor for advanced glycation end products (RAGE)-independent signaling pathways and regulating mitochondrion-mediated apoptosis

Primary antibody Dilution Source Secondary antibody (dilution, source)
Phospho-p38 (Thr180/Tyr182) rabbit mAb 1:800 Cell Signaling Technology (CST) Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)
Phospho-MK2 (Thr334) rabbit mAb 1:200 CST Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)
Phospho-HSP27 (Ser82) rabbit mAb 1:50 CST Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)
Phospho-SAPK/JNK (Thr183/Tyr185) mouse mAb 1:400 CST Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)
Phospho-c-Jun (Ser73) mouse mAb 1:100 CST Alexa Fluor 555 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)
Phospho-p44/42 MAPK (Thr202/Tyr204) mouse mAb 1:200 CST Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)
Anti-NFκB p65 rabbit mAb 1:200 CST Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)
Anti-Bcl-2 rabbit mAb 1:200 CST Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA)
Anti-cytochrome c mouse mAb 1:400 Santa Cruz Biotechnology Alexa Fluor 550 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA)