Table 1 Primary antibodies and secondary antibodies used in this study
Primary antibody | Dilution | Source | Secondary antibody (dilution, source) |
|---|---|---|---|
Phospho-p38 (Thr180/Tyr182) rabbit mAb | 1:800 | Cell Signaling Technology (CST) | Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA) |
Phospho-MK2 (Thr334) rabbit mAb | 1:200 | CST | Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA) |
Phospho-HSP27 (Ser82) rabbit mAb | 1:50 | CST | Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA) |
Phospho-SAPK/JNK (Thr183/Tyr185) mouse mAb | 1:400 | CST | Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA) |
Phospho-c-Jun (Ser73) mouse mAb | 1:100 | CST | Alexa Fluor 555 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA) |
Phospho-p44/42 MAPK (Thr202/Tyr204) mouse mAb | 1:200 | CST | Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA) |
Anti-NFκB p65 rabbit mAb | 1:200 | CST | Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA) |
Anti-Bcl-2 rabbit mAb | 1:200 | CST | Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, Carlsbad, CA, USA) |
Anti-cytochrome c mouse mAb | 1:400 | Santa Cruz Biotechnology | Alexa Fluor 550 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA) |