Figure 1

Reactive oxygen species production and autophagy in periodontitis patients. (A) ROS production was analyzed in PBMCs from patients with and without periodontitis by flow cytometry as described in Methods. *P < 0.001 periodontitis versus no periodontitis. (B). Expression of LC3 transcripts in PBMCs from patients with and without periodontitis assessed by real-time PCR as described in Methods.*P <0.001 periodontitis versus no periodontitis. (C) Correlation between ROS levels and MAP-LC3 mRNA levels in PBMCs from patients with periodontitis. Data represent the mean ± SD of three separate experiments. non perio: participants without periodontitis; PBMC: peripheral blood mononuclear cells; perio: patients with periodontitis; ROS: reactive oxygen species; SD: standard deviation.