Figure 6
From: Insufficient maintenance DNA methylation is associated with abnormal embryonic development
The effect of DNMT1 inhibition on embryonic attachment in vitro. A: Blastulas collected from ICR mice were pre-treated with or without DNMT1 inhibitor and placed on an Ishikawa cell-covered plate. Erosion of Ishikawa cells occurred after implantation (The bar represents 100 μm). B: Pretreatment with DNMT1 inhibitor significantly decreased the implantation rate of embryos in vitro. The rate of embryo attachment to Ishikawa cells was 30/71 (42%) and 55/71 (77%) at 48 hours and 72 hours, respectively, for the control group. The rate decreased to 18/71 (25%) and 43/71 (61%) at 48 hours and 72 hours, respectively, in the procainamide treatment group (* P < 0.05). C: The presence of DNMT1 inhibitor did not affect the LIF expression in Ishikawa cells. LIF, leukemia inhibitory factor.