Table 1 Human gene sequences for IL-1α and GAPDH, PCR annealing temperatures and number of amplification cycles
From: Apolipoprotein epsilon 3 alleles are associated with indicators of neuronal resilience
Gene analyzed | Human sequences | Annealing temp. (°C) | Cycle no. |
|---|---|---|---|
Gel-based PCR | |||
IL-1α | F: AAG CCT TCC TGC CGC AAC | 57 | 32 |
R: CTG CAC CTA CCA AAC ACG G | |||
GAPDH | F: AGG TCG GAG TCA ACG GAT TTG | 57 | 32 |
R: TGG CAG GTT TTT CTA GAC GGC | |||