Western blot analysis of the mitochondrial proteins UCP-2 and PGC1α as well as the Ca
2+ regulatory proteins SERCA2a, Na
2+ exchanger and phospholamban in myocardium from FVB and ALDH2 mice treated with or without streptozotocin. (A) Representative gel blots of UCP-2, PGC1α, SERCA2a, Na+-Ca2+ exchanger, phospholamban and GAPDH (loading control) using specific antibodies; (B) UCP-2; (C) PGC1α; (D) SERCA2a; (E) Na+-Ca2+ exchanger; (F) phospholamban. All proteins were normalized to the loading control GAPDH. Mean ± SEM, n = 5 to 6 mice per group. *P < 0.05 versus FVB group; #
P < 0.05 versus FVB-STZ group.