Table 2 Example of the REMARK profile illustrated using data from a study of ploidy in patients with advanced ovarian cancer [157] (from [20]).

Marker (If non-binary: how was marker analyzed? continuous or categorical. If categorical, how were cutpoints determined?)

M = ploidy (diploid, aneuploid)

Further variables

(variables collected, variables available for analysis, baseline variables, patient and tumor variables)

v1 = age, v2 = histologic type, v3 = grade, v4 = residual tumor, v5 = stage, v6 = ascites^a, v7 = estrogen^a, v8 = progesterone^a, v9 = CA-125^a

Patients

n

Remarks

Assessed for eligibility

257

Disease: Advanced ovarian cancer, stage III and IV

Patient source: Surgery 1982 to 1990, University Hospital Freiburg

Sample source: Archived specimens available

Excluded

73

General exclusion criteria^b, non-standard therapy^b, coefficient of variation > 7%^b

Included

184

Previously untreated.

Treatment: all had platinum based chemotherapy after surgery

With outcome events

139

Overall survival: death from any cause

b) Statistical analyses of survival outcomes

Analysis

Patients

Events

Variables considered

Results/remarks

A1: Univariable

184

139

M, v1 to v5

Table 2, Figure 1

A2: Multivariable

174

133

M, v1, v3 to v5

Table 3 [v2 omitted because many missing data; Backward selection, see text]

A3: Effect for ploidy adjusted for v4

184

139

M, v4

Figure 2 [Based on result of A2]

A4: Interaction: ploidy and stage

175

133

M, v1, v2, v4, v5

See text

A5: Ploidy in stage subgroups

128

88

M

Figure 3

56

51

M

Figure 4