Functional parameters of hepatocyte-like cells differentiated from hPMSCs. (A-D): glycogen storage capacity. (A) Primary human hepatocytes as a positive control (10×); (B) hPMSCs cultured in normal growth medium did not display positive PAS staining (4×); (C, D) hPMSCs cultured in hepatogenic differentiation medium for 28 days were positively stained, revealing cytoplasmic glycogen accumulation (C, 4×; D, 10×). (E-H) LDL uptake. (E) Primary human hepatocytes served as a positive control (10×); (F) hPMSCs maintained in growth medium were stained negatively; (G, H) LDL uptake was detected in hepatocyte-like cells 21 days after differentiation (G, 10×; H, 20×). (I) urea synthesis assay. After hepatogenic differentiation culture for 28 days, urea levels in culture supernatants were significantly increased. (J) CYP-450 enzymatic activities were determined. After hepatogenic differentiation culture for 35 days, CYP-450 enzymatic activity was significantly higher. Data are mean ± SD (n = 4, *P < 0.05). But urea synthesis and CYP3A4 enzymatic activities of the hepatocyte-like cells derived from hPMSCs were significantly lower than those of the primary human hepatocytes (**P < 0.01).