Table 1 Primers and conditions used for RT-PCR
| Primer | Sequence | Amplicon size (bp) | Cycle |
|---|---|---|---|
| ALB |
5'- CACAGTTGCAACTCTTCGTGAAAC-3' 5'- AGCAGTGCACATCACATCAACC-3 | 154 | 94°C/30 s; 55°C/40 s; 72°C/60 s |
| CK18 |
5'- CACCGTCGTCCGCAAAGCCT-3' 5'- CCTGCCAGACCCCCGGCTAT-3 | 269 | 94°C/30 s; 58°C/40 s; 72°C/60 s |
| CK19 |
5'-AGGTGGATTCCGCTCCGGGCA-3' 5'- ATCTTCCTGTCCCTCGAGCA-3' | 460 | 94°C/60 s; 64°C/60 s; 72°C/120 s |
| AFP |
5'- GCATGTGCAGTAATGAAAAAT-3' 5'- GAACAAAACTTGCCAAGAAGAT-3' | 400 | 94°C/30 s; 50°C/40 s; 72°C/60 s |
| β-actin |
5'- GAGCGGGAAATCGTGCGTGACATT-3' 5'- GATGGAGTTGAAGGTAGTTTCGTG-3' | 234 | 94°C/30 s; 58°C/40 s; 72°C/60 s |
| LPL |
5'-ATGGAGAGCAAAGCCCTGCTC-3' 5'-TACAGGGCGGCCACAAGTTTT-3' | 298 | 94°C/30 s; 56°C/30 s; 72°C/60 s |
| OPN |
5'-CTAGGCATCACCTGTGCCATACC-3' 5'-CTAGGCATCACCTGTGCCATACC-3' | 330 | 94°C/30 s; 55°C/45 s; 72°C/60 s |
