Table 1 Primers and conditions used for RT-PCR
Primer | Sequence | Amplicon size (bp) | Cycle |
|---|---|---|---|
ALB | 5'- CACAGTTGCAACTCTTCGTGAAAC-3' 5'- AGCAGTGCACATCACATCAACC-3 | 154 | 94°C/30 s; 55°C/40 s; 72°C/60 s |
CK18 | 5'- CACCGTCGTCCGCAAAGCCT-3' 5'- CCTGCCAGACCCCCGGCTAT-3 | 269 | 94°C/30 s; 58°C/40 s; 72°C/60 s |
CK19 | 5'-AGGTGGATTCCGCTCCGGGCA-3' 5'- ATCTTCCTGTCCCTCGAGCA-3' | 460 | 94°C/60 s; 64°C/60 s; 72°C/120 s |
AFP | 5'- GCATGTGCAGTAATGAAAAAT-3' 5'- GAACAAAACTTGCCAAGAAGAT-3' | 400 | 94°C/30 s; 50°C/40 s; 72°C/60 s |
β-actin | 5'- GAGCGGGAAATCGTGCGTGACATT-3' 5'- GATGGAGTTGAAGGTAGTTTCGTG-3' | 234 | 94°C/30 s; 58°C/40 s; 72°C/60 s |
LPL | 5'-ATGGAGAGCAAAGCCCTGCTC-3' 5'-TACAGGGCGGCCACAAGTTTT-3' | 298 | 94°C/30 s; 56°C/30 s; 72°C/60 s |
OPN | 5'-CTAGGCATCACCTGTGCCATACC-3' 5'-CTAGGCATCACCTGTGCCATACC-3' | 330 | 94°C/30 s; 55°C/45 s; 72°C/60 s |