Skip to main content

Table 1 Primers and conditions used for RT-PCR

From: Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure

Primer Sequence Amplicon size (bp) Cycle
ALB 5'- CACAGTTGCAACTCTTCGTGAAAC-3'
5'- AGCAGTGCACATCACATCAACC-3
154 94°C/30 s; 55°C/40 s; 72°C/60 s
CK18 5'- CACCGTCGTCCGCAAAGCCT-3'
5'- CCTGCCAGACCCCCGGCTAT-3
269 94°C/30 s; 58°C/40 s; 72°C/60 s
CK19 5'-AGGTGGATTCCGCTCCGGGCA-3'
5'- ATCTTCCTGTCCCTCGAGCA-3'
460 94°C/60 s; 64°C/60 s; 72°C/120 s
AFP 5'- GCATGTGCAGTAATGAAAAAT-3'
5'- GAACAAAACTTGCCAAGAAGAT-3'
400 94°C/30 s; 50°C/40 s; 72°C/60 s
β-actin 5'- GAGCGGGAAATCGTGCGTGACATT-3'
5'- GATGGAGTTGAAGGTAGTTTCGTG-3'
234 94°C/30 s; 58°C/40 s; 72°C/60 s
LPL 5'-ATGGAGAGCAAAGCCCTGCTC-3'
5'-TACAGGGCGGCCACAAGTTTT-3'
298 94°C/30 s; 56°C/30 s; 72°C/60 s
OPN 5'-CTAGGCATCACCTGTGCCATACC-3'
5'-CTAGGCATCACCTGTGCCATACC-3'
330 94°C/30 s; 55°C/45 s; 72°C/60 s