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Figure 9 | BMC Medicine

Figure 9

From: Antitumor activity of phenethyl isothiocyanate in HER2-positive breast cancer models

Figure 9

Phenethyl isothiocyanate (PEITC) enhances the cytotoxic effects of doxorubicin. (A) MDA-MB-231 cells were treated with PEITC (4 μM) and/or doxorubicin (3 μM) for 24 h and analyzed by sulforhodamine B cell survival assay. (B) MDA-MB-231 cells were treated with PEITC (4 μM) and/or doxorubicin (3 μM) for 24 h and analyzed by western blotting for HER2 and cleavage of caspase 3 and poly-ADP ribose polymerase (PARP). (C) MDA-MB-231 (high HER2 (HH)) cells were treated with 4 μM of PEITC and 3 μM of doxorubicin for 24 h and analyzed by sulforhodamine B cell survival assay. (D) The cell lysates from MDA-MB-231 (HH) cells treated with 4 μM of PEITC and/or 3 μM of doxorubicin were analyzed by western blotting for HER2, cleaved caspase 3 and PARP. Actin was used as internal loading control. Statistically different when compared with control (P < 0.05).

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