Figure 1

Diindolylmethane (DIM) induces apoptosis in ovarian cancer cells. (A) SKOV-3, OVCAR-3, TOV-21G, A2780, OVCAR-429 and OVCAR-433 cells were treated with or without 50 μM or 75 μM DIM for 24 h. Cells that were positive for annexin or propidium iodide (PI) or both were measured using flow cytometry. Representative images are shown. (B) Representative western blots of cleaved caspase 3 and cleaved poly(ADP-ribose) polymerase (PARP) from the lysates collected from (i) SKOV-3, (ii) OVCAR-3, (iii) TOV-21G, (iv) A2780, (v) OVCAR-429 and (vi) OVCAR-433 cells treated with or without 75 μM DIM.