Identification of hAFSCs in situ with IHC staining. (A) HuNu expression of hAFSCs in vitro (200×). (B) Localization of the injected hAFSCs. At Day 3, the injected cells were detected focally at the injection site. They migrated into surrounding tissue from Day 5 and cell signals were gradually decreased from Day 7 (200×). Ctrl (-), negative control without cell treatment.