Anatomical, histological and IHC analysis of regenerated sphincters. (A) Anatomic location of the urethral sphincter and cell injection. Location of the urethral sphincter on mouse model (upper) and cell injection on the urethral sphincter area using a Hamilton microsyringe (lower). (B) The sphincter of hAFSCs-injected animals showed apparently normal muscle regeneration with strong MyoD and myosin heavy chain (MHC) expression. The Cell (-) group showed atrophic sphincters. Ctrl, positive control with sham operation; Cell (-), pudendal neurectomy without cell injection; Cell (+), pudendal neurectomy with hAFSCs injection (200×).