Table 1 Primer sequences for real-time PCR
Gene | Sequences |
|---|---|
PAX7 | 5'-GCAAATTGCTGTCCTGCTCA 5'-TGAAAACTGGTCACATCTGCCT |
MYF5 | 5'-ACCGATTCACAGCCTCGAACT 5'-TGTGTATTAGGCCCTCCTGGAA |
MYOD | 5'-ACAGCGCGGTTTTTTCCAC 5'-AACCTAGCCCCTCAAGGTTCAG |
MYOGENIN | 5'-TGGCAGGAACAAGCCTTTTC 5'-ACAGGCAGGTAGTTTTCCCCA |
MEF2 | 5'-ATTCCACCAGGCAGCAAGAA 5'-GGAGTTGCTACGGAAACCACTG |
MLP | 5'-AAGGCTCTTGACAGCACGACAG 5'-TGTCCATACCCGATCCCTTTG |
β-ACTIN | 5'-ATCGTCCACCGCAAATGCT 5'-AAGCCATGCCAATCTCATCTTG |
Pax7 | 5'-ACCAAGCTTTCAAGTCCGCA 5'-GCCTTACATTCTGGAGGATGGA |
Myf | 5'-CTCTGAAGGATGGACATGACGG 5'-ACTGGTCCCCAAACTCATCCTC |
MyoD | 5'-TTCCGGAGTGGCAGAAAGTTAA 5'-TCAAGTCTATGTCCCGGAGTGG |
Myogenin | 5'-TATCCGGTTCCAAAGCCTCTG 5'-GCGGCAGCTTTACAAACAACA |
Mef2 | 5'-AACCCCAATCTTCTGCCACTG 5'-ATCAGACCGCCTGTGTTACCTG |
Mlp | 5'-GCTGAACAAGTTACTGAGCGGC 5'-ATTTTGCACCTCCACCCCA |
Gapdh | 5'-TGT GTCCGTCGTGGATCTGA 5'-CCTGCTTCACCACCTTCTTGA |