TLR4 expression in different HCC cells lines. (A) qPCR was used to detected TLR4 mRNA expression in HepG2, Huh7, Hep3B and SMMC-7721 cells (*P < 0.05). (B) FACS analysis for TLR4 protein in HCC cell lines untreated or treated with LPS (10 μg/ml) for 48 hours. The data shown in A and B are from one representative experiment of three performed. HCC, hepatocellular carcinoma; LPS, lipopolysaccharide; TLR4, toll-like receptor 4.