Table 2 TaqMan® gene expression assays used to amplify the pluripotency-related genes
Gene | Assay ID | Amplicon size, nucleotides | Dye |
|---|---|---|---|
NANOG | Hs02387400_g1 | 109 | FAM |
SOX2 | Hs01053049_s1 | 91 | FAM |
OCT4 | Hs00742896_s1 | 65 | FAM |
KLF4 | Hs00358836_m1 | 110 | FAM |
REX1 | Hs00358836_m1 | 102 | FAM |
18S | 4352930E | 187 | FAM/MGB |