Figure 5
Transgenerational F3 generation sperm epimutations. (a) Chromosomal locations for differential DNA methylated regions (DMR) in sperm DNA from F3 generation dichlorodiphenyltrichloroethane (DDT) lineage rats compared to control lineage (arrowheads). The high stringency intersection epimutations are identified as red arrows, which is a subset of the less stringent ‘average’ DMR indicated with open arrows. The chromosomal size and number are listed (Additional file 8: Table S6 and Additional file: 9 Table S7). (b) A Venn diagram of DMR from various F3 generation exposure lineages including: vinclozolin, plastics, pesticides, hydrocarbons and DDT. The total number of DMR per exposure lineage in brackets is presented and unique and overlapping DMR identified. (c) The CpG/100 bp is presented and the corresponding number of DMR associated. The density is presented as the number of CpG for 100 bp of the DMR.