Figure 2

FLB translocation in DLN following injection in TA muscle. a) Marked translocation of FLBs in parafollicular areas of popliteal DLNs (d4); b) Flow cytometry showing that most FLB-loaded cells extracted from DLN express CD11c at either an intermediate or strong level (d4); c) Immunocytochemistry on CD11b⁺ cells extracted from DLNs were usually Gr1⁺/Ly6C⁺, especially when they had ingested a few particles (left), whereas heavily loaded ones were often Gr1^-/Ly6C^- (right); d) The number of FLB-loaded cells peaked at d4 post-injection in both popliteal and inguinal DLNs; e) The migration inhibitor BW245C co-injected with FLBs in muscle markedly decreased the number of FLB-loaded cells detected in DLNs at d4 post-injection. The effect was more pronounced in the downstream inguinal DLN; f) The migration inhibitor BW245C co-injected with FLBs in muscle markedly decreased the number of FLB-loaded cells detected in DLNs at d4 post-injection. The effect was more pronounced in the downstream inguinal DLN; (histograms: n = 3 per group, mean + SD, * P <0.05, ** P <0.01, *** P <0.005; bars: 100 μm [a]; 5 μm [c]). d, day; DLNs, draining lymph nodes; FLBs, fluorescent latex beads; TA, tibialis anterior.