FLB biodistribution in spleen and blood following injection in TA muscle. a) The number of FLB-loaded cells peaked at d21 in spleen; b) In spleen, FLBs were detected in CD11b+ cells as assessed by immunohistochemistry (left) or after cell sorting (right); c) On blood smears, most FLBs were cell-associated from d4 and peaked at d21 post injection; circulating FLB-loaded cells were still detected at d90 endpoint; d) Circulating FLB-loaded cells were CD11b+ (d21); e,f) GR1/Ly6C immunophenotyping of CD11b+ cells that have ingested FLBs. Most are Gr1+/Ly6C+ both in DLNs at d4 (a) and in spleen at d21 (b). (histograms n = 3 per group, mean ± SD, * P <0.05, ** P <0.01, *** P <0.005; bars: 5 μm). d, day; FLB, fluorescent latex beads; n, number; TA, tibialis anterior.