Figure 8From: Slow CCL2-dependent translocation of biopersistent particles from muscle to brain Biodistribution of Al particles. a) Morin stain for aluminum shows rounded alum cytoplasmic agglomerates within muscle macrophages after i.m. vaccine administration in C57 mouse; b) Morin stain confirms that phagocytosed Al-Rho nanohybrids are associated with Al and form particles similar in size to alum agglomerates; c-e) Al-Rho nanohybrids show a time-dependent distribution in DLNs, spleen, and brain strikingly similar to that of FLBs; f) Al-Rho injected by the s.c. route translocate to DLNs and spleen, as observed with the i.m. route; g,h) Ly6C immunophenotyping of CD11b+ cells that have ingested Al-Rho: most are Gr1+/Ly6C+ both in DLNs at d4 (g) and spleen at d21 (h). (histograms: n = 3 per group, mean ± SD, * P <0.05, ** P <0.01, *** P <0.005; bar in a: 10 μm). d, day; DLN, draining lymph nodes; FLBs, fluorescent latex beads; n, number.Back to article page