Figure 6From: Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis AP5 immunofluorescence staining of differentiated human podocytes incubated with different additional materials. A. AP5 antibody replaced by phosphate-buffered saline; B. Podocytes incubated with bovine serum albumin; C. Podocytes incubated with RPMI 1640; D. Podocytes incubated with RPMI 1640 combined with blocking antibody; E. Podocytes incubated with normal urine; F. Podocytes incubated with normal urine combined with blocking antibody; G. Podocytes incubated with recombinant human suPAR protein; H. Podocytes incubated with recombinant human suPAR protein combined with blocking antibody; I. Podocytes incubated with urine from patients with primary FSGS; J. Podocytes incubated with urine from patients with primary FSGS combined with blocking antibody; K. Podocytes incubated with urine from a patient with minimal change disease; L. Podocytes incubated with urine from a patient with minimal change disease combined with blocking antibody; M. Podocytes incubated with urine from a patient with membranous nephropathy; N. Podocytes incubated with urine from a patient with membranous nephropathy combined with blocking antibody. FSGS, focal segmental glomerulosclerosis; suPAR, soluble urokinase receptor.Back to article page