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Figure 2 | BMC Medicine

Figure 2

From: Evolution of somatic mutations in mammary tumors in transgenic mice is influenced by the inherited genotype

Figure 2

ERK phosphorylation in MMTV-Wnt1 tumors with and without H-Ras mutations. (A) Protein lysates from tumors from a MMTV-v-H-Ras mouse (first lane) and MMTV-Wnt1 mice with and without activating H-Ras mutations were subjected to electrophoresis in 10% polyacrylamide gels, transferred to a nitrocellulose membrane and then exposed to antibodies against phospho-ERK, total ERK and γ-tubulin as described in Methods. (B) Primary tumor sections from (a) MMTV-v-H-Ras mouse and (b) MMTV-Wnt1 mice without activating H-Ras mutations and (c,d) with activating H-Ras mutations were incubated with antibodies against phospho-ERK as described in Methods.

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