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Table 1 Primers

From: Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model

Template Primers Product Length Melting Temperature Hot Measurement Fidelity n
18S gag gtt cga aga cga tca ga (sense) 316 bp 83.2 ± 02°C 78°C 1.89 ± 0.02 17
  tcg ctc cac caa cta aga ac (antisense)      
KCNJ10 tgg tgt ggt gtg gta tct gg (sense) 411 bp 83.2 ± 02°C 78°C 1.86 ± 0.02 15
  tga agc agt ttg cct gtc ac (antisense)      
KCNQ1 ttt gtt cat ccc cat ctc ag (sense) 239 bp 82.5 ± 02°C 79°C 1.85 ± 0.02 17
  gtt gct ggg tag gaa gag (antisense)      
KCNQ4 ccc gga aac cct tct gtg tc (sense) 245 bp 83.2 ± 02°C 80°C 1.87 ± 0.01 17
  aaa gat gag cac cag gaa cc (antisense)