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Table 2 Mouse plasma and tissue sterol analyses

From: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol

Plasma (mg/dL)
Group Cholesterol Campesterol Stigmasterol β-Sitosterol
Abcg8+/+ 73.0 ± 14 1.1 ± 0.7 ND 0.5 ± 0.5
Abcg8+/- 54.0 ± 10* 1.8 ± 0.3* ND 0.8 ± 0.2*
Abcg8-/- 35.0 ± 6.0* 8.8 ± 2.1* ND 18.5 ± 5.0*
Liver (μg/g wet tissue)
Group Cholesterol Campesterol Stigmasterol β-Sitosterol
Abcg8+/+ 2280 ± 310 52.2 ± 15.0 ND 16.7 ± 8.6
Abcg8+/- 2460 ± 588 76.4 ± 12.3* 1.6 ± 3.3* 26.7 ± 6.4*
Abcg8-/- 1202 ± 264* 233.9 ± 48.1* 22.7 ± 6.3* 376.3 ± 91.6*
Spleen (μg/g wet tissue)
Group Cholesterol Campesterol Stigmasterol β-Sitosterol
Abcg8+/+ 2702 ± 310 41.7 ± 7.6 15.1 ± 14.1 21.9 ± 10.9
Abcg8+/- 2578 ± 320 66.8 ± 18.8* 14.5 ± 16.9* 32.2 ± 6.1*
Abcg8-/- 1779 ± 195* 353.8 ± 97.5* ND* 436.6 ± 148.5*
Brain (μg/g wet tissue)
Group Cholesterol Plant sterols Desmosterol Lathosterol
Abcg8+/+ 11301 ± 156 10.2 ± 3.9 102.0 ± 13.3 52.0 ± 0.3
Abcg8+/- 11846 ± 814 24.5 ± 5.5 113.7 ± 25.5 49.1 ± 4.2
Abcg8-/- 11846 ± 1061 63.0 ± 6.5 123 ± 18.3 56.9 ± 9.5
  1. Animals used in these studies were 12 weeks of age, a mixture of male and female and fed a regular chow diet. *P < 0.05 for -/- versus +/+ and -/- versus +/-. ND, not detectable.