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Table 2 Mouse plasma and tissue sterol analyses

From: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol

Plasma (mg/dL)

Group

Cholesterol

Campesterol

Stigmasterol

β-Sitosterol

Abcg8+/+

73.0 ± 14

1.1 ± 0.7

ND

0.5 ± 0.5

Abcg8+/-

54.0 ± 10*

1.8 ± 0.3*

ND

0.8 ± 0.2*

Abcg8-/-

35.0 ± 6.0*

8.8 ± 2.1*

ND

18.5 ± 5.0*

Liver (μg/g wet tissue)

Group

Cholesterol

Campesterol

Stigmasterol

β-Sitosterol

Abcg8+/+

2280 ± 310

52.2 ± 15.0

ND

16.7 ± 8.6

Abcg8+/-

2460 ± 588

76.4 ± 12.3*

1.6 ± 3.3*

26.7 ± 6.4*

Abcg8-/-

1202 ± 264*

233.9 ± 48.1*

22.7 ± 6.3*

376.3 ± 91.6*

Spleen (μg/g wet tissue)

Group

Cholesterol

Campesterol

Stigmasterol

β-Sitosterol

Abcg8+/+

2702 ± 310

41.7 ± 7.6

15.1 ± 14.1

21.9 ± 10.9

Abcg8+/-

2578 ± 320

66.8 ± 18.8*

14.5 ± 16.9*

32.2 ± 6.1*

Abcg8-/-

1779 ± 195*

353.8 ± 97.5*

ND*

436.6 ± 148.5*

Brain (μg/g wet tissue)

Group

Cholesterol

Plant sterols

Desmosterol

Lathosterol

Abcg8+/+

11301 ± 156

10.2 ± 3.9

102.0 ± 13.3

52.0 ± 0.3

Abcg8+/-

11846 ± 814

24.5 ± 5.5

113.7 ± 25.5

49.1 ± 4.2

Abcg8-/-

11846 ± 1061

63.0 ± 6.5

123 ± 18.3

56.9 ± 9.5

  1. Animals used in these studies were 12 weeks of age, a mixture of male and female and fed a regular chow diet. *P < 0.05 for -/- versus +/+ and -/- versus +/-. ND, not detectable.