Figure 1From: STAT1-dependent expression of energy metabolic pathways links tumour growth and radioresistance to the Warburg effect Knockdown of STAT1 in SCC61 causes tumour growth suppression and radio sensitization. In untreated tumours, STAT1 [Signal Transducer and Activator of Transcription 1] knockdown (KD/C; white diamond) significantly reduced day 40 tumour volume by 2.7-fold compared to a control-vector transfected STAT1 wild-type (WT/C; black diamond) (mean ± standard error of mean [SEM] [mm3]: WT/C = 1154 ± 404, KD/C = 427 ± 130; Student's t-test P = 0.050). Ionizing radiation (IR) was delivered in 5 Gy fractions on days 0-5 (total 30 Gy). In irradiated tumours, KD of STAT1 (KD/IR; white triangle) significantly suppressed day 68 tumour volume by 6.5-fold compared to that of a STAT1 WT tumour (WT/IR; black triangle) (mean ± SEM [mm3]: WT/IR = 738 ± 232, KD/IR = 114 ± 21.2; Student's t-test P = 0.017). Point, mean tumour volume; error bars, SEM. Tumour volumes are shown on a semi-log scale.Back to article page