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Table 5 Tandem mass spectrometric results of various standards

From: Reduced levels of hydroxylated, polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients: implications for early screening and detection

Standard 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
[M-H]-(%) 335 (45%) 319 (100%) 319 (100%) 429 (14%) 415 (28%) 445 (90%) 445 (68%) 429 (100%) 445 (64%) 397 (6%) 385 (2%) 397 (5%) 451 (28%) 287 (1%) 431 (100%)
*Chain cut ions (%) 219 (15%) 219 (80%) 203 (3%)             
   201 (1%) 203 (15%) 163 (25%)             
   115 (100%) 175 (55%) 155 (60%)             
    113 (20%) 127 (10%)             
     111 (5%)             
*Peripheral cut ions (%) Loss of H 2 O 317 (16%) 301 (60%) 301 (55%)    427 (50%)   401 (30%) 401 (28%) 379 (10%) 367 (18%) 379 (5%)   269 (1%) 413 (1%)
  Loss of 2H 2 O 299               
  Loss of CO 2 291 (2%) 275 (20%) 275 (4%)    401 (1%) 401 (35%)         
  Loss of CO 2 and H 2 O 273 (6%) 257 (70%) 257 (80%)             
  *Loss of CO 2 and 2H 2 O                
  *Loss of 3H 2 O                
Secondary daughter ions (%) 189 (1%) 167 (5%) 291 (1%) 414 (5%) 400 (78%) 295 (70%) 386 (52%) 163 (100%) 386 (43%) 309 (5%) 273 (3%) 295 (5%) 436 (10%) 187 (5%) 399 (100%)
   163 (1%) 149 (2%) 171 (1%) 163 (100%) 175 (13%) 149 (90%) 179 (60%) 135 (20%) 179 (50%) 213 (15%) 259 (25%) 253 (6%) 241 (21%) 173 (10%) 393 (40%)
   145 (2%) 121 (20%) 107 (1%) 135 (8%) 149 (100%) 136 (100%) 135 (100%) 218 (5%) 166 (20%) 201 (30%) 255 (15%) 211 (12%) 227 (62%) 159 (18%) 373 (40%)
   99 (1%) 99 (1%) 59 (1%)   121 (90%) 121 (20%) 107 (25%) 123 (5%) 135 (100%) 173 (37%) 213 (20%) 159 (20%) 223 (48%) 145 (28%) 355 (40%)
   95 (1%) 59 (1%)        122 (25%) 159 (35%) 173 (47%) 161 (15%) 213 (55%) 119 (15%) 337 (20%)
   71 (1%)         107 (25%) 107 (90%) 161 (52%) 147 (20%) 199 (57%) 105 (20%) 223 (40%)
   59 (1%)          81 (68%) 159 (75%) 107 (25%) 187 (100%) 95 (36%) 85 (40%)
            69 (100%) 149 (40%) 105 (15%) 185 (52%) 93 (28%)  
             147 (72%) 95 (38%) 171 (55%) 81 (58%)  
             107 (100%) 93 (17%) 71 (86%) 69 (100%)  
             81 (82%) 83 (25%)    
              81 (40%)    
              36 (100%)    
  1. *This terminology is specific to fatty acid fragmentation.
  2. 5S,6S-(7E,9E,11Z,14Z)-dihydroxyeicosatetraenoic acid (1), 15S-Hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (2) and 8R-Hydroxy-(5Z,9E,11Z,14Z)-eicosatetraenoic acid (3) (Table 6), α-tocopherol (4) γ-tocopherol (5), 13-(6-hydroxy-2,7,8-trimethylchroman-2-yl)-2,6,10-trimethyltridecanoic acid (6), 16-(4,5-dimethyl-3,6-dioxo cyclohexa-1,4-dienyl)-2,6,10,14-tetramethylhexadecanoic acid (7), 6-hydroxy-2,7-dimethyl-2-(4,8,12-trimethyltridecyl)chroman-8-carbaldehyde (8), 6-hydroxy-2,7-dimethyl-2-(4,8,12-trimethyltridecyl)chroman-8-carboxylic acid (9), calciferol (10), cholecalciferol (11), ergosterol (12), phylloquinone (13), retinol (14) and 3β,7α-dihydroxy-5-cholestenoic acid (15).
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