Mitf-Mdel, a novel melanocyte/melanoma-specific isoform of microphthalmia-associated transcription factor-M, as a candidate biomarker for melanoma

Table 1 Primer sequences for mRNA analysis by real-time polymerase chain reaction.

Gene	Primers (human)	Amplicon	Tm
GADPH	F: 5'-cgagatccctccaaaatcaa-3';	170	60
	R: 5'-ttcacacccatgacgaacat-3'
MITF-M	F: 5'-ttatagtaccttctctttgccagtcc-3'	146	52
	R: 5'-gtttatttgctaaagtggtagaaaggtact-3'
MITF-Mdel	F: 5'-ttatagtaccttctctttgccagtcc-3';	120	52
	R: 5'-cttataaaatccctgccgttgg-3'

ISSN: 1741-7015