Oligonucleotide | Position (exon) | 5' Position | Sequence (5' to 3') | Primer/probe concentrations (nM) | Amplicon size |
---|---|---|---|---|---|
5 | 518 | F: GTACCCTAACTAGCCGAGGAAGAA | 300 | ||
6 | 597 | R: GTGAATGTGGCCTGTGCAGT | 300 | ||
HIF-1α (total) | 5 to 6 junction | 544 | P: ATGAACATAAAGTCTGCAACATGGAAGGTATTG | 200 | 80 bp |
1 | 17 | F: GCGCGAACGACAAGAAAAATAG | 50 | ||
2 | 138 | R: TGGCAACTGATGAGCAAGCT | 300 | ||
HIF-1α TAG | 2 | 74 | P: ATGCAGCCAGATCTCGGCGAAGTAAA | 200 | 122 bp |
13 to 15 junction | 2,182 | F: TCACTTTTTCAAGCAGTAGGAATTATTTAG | 600 | ||
15 | 2,330 | R: TAATTCTTCACCCTGCAGTAGGTTT | 600 | ||
HIF-1α 736 | 15 | 2,260 | P: TGACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGG | 200 | 149 bp |
11 | 1,566 | F: TGTGGATAGTGATATGGTCAATGAATT | 300 | ||
13 | 1,699 | R: TAGTATCTTTGGATTTAGTTCTTCCTCAGG | 300 | ||
HIF-1α 557 | 11 to 13 junction | 1,642 | P: AAGCAAACCCATTTTCTACTCAGAACTACA | 200 | 134 bp |
10 | 1,490 | F: AGACACCTAGTCCTTCCGATGG | 600 | ||
13 | 1,580 | R: AAGCTAGTATCTTTGGATTTAGTTCTTCCT | 600 | ||
HIF-1α 516 | 10 to 13 junction | 1,513 | P: AGCACTAGACAAAGTTCACCTGAGAACTACAGT | 200 | 91 bp |