Figure 4

Suppression of lamin A/C expression in ovarian surface epithelial cells leads to a deformed nuclear shape. Primary HOSE cells were treated with siRNA to lamin A/C for three days and were analyzed for the effect on nuclear size and morphology. (A) Western blot shows the specific suppression of protein by siRNA to emerin, lamin A/C, and lamin B1 in primary HOSE cells. (B) A low magnification image (× 200) shows the presence of a high percentage of cells with large and deformed nuclear morphology following suppression of lamin A/C by siRNA in primary HOSE cells for three days. Suppression of lamin B had no significant effect on nuclear morphology. (C) Examples shown at high magnification (× 1,000) of individual deformed nuclei with reduced and patchy lamin A/C staining. (D) The nuclear morphological abnormality was scored by computer-assisted image analysis. About 100 nuclei in each group treated by siRNA were analyzed and the percentage of normal and atypical nuclear morphology is shown. ScControl: scrambled controls. (E) The nuclear size/diameter was scored by computer-assisted image analysis (AxioVision software 4) following shRNA treatment for 3 days. Nuclear sizes were plotted following computer-assisted imaging analysis of 50 nuclei randomly selected from each group, compared to OVCAR5 ovarian cancer cells.