Figure 5

Lamin A/C suppression in primary ovarian epithelial cells results in increased nuclear size, aneuploidy, and polyploidy. Primary HOSE cells were transfected with control or shRNA to suppress lamin A/C and were subjected to analysis for DNA content and ploidy. (A) The shRNA-treated and control HOSE cells were sorted by flow cytometry to compare DNA content. Two aneuploid ovarian cancer cell lines, OVCAR5 (51 to 57 chromosomes per cell) and OVCAR3 (52 to 70 chromosomes per cell) were analyzed for comparison. A subtle increase in DNA content was observed in lamin A/C-suppressed HOSE cells. (B) In situ hybridization with X chromosome centromere probe of control HOSE cells treated with scrambled shRNA. Two representative fields are shown. (C) In situ hybridization with X centromere probe of lamin A/C-suppressed HOSE cells. Six representative fields are shown for the presence of 3, 4, and other multiple X-chromosome-containing nuclei.