Figure 4

The expression of two stem cell markers STRO-1 and Oct-4. As seen, hACL-SCs stained more weakly for STRO-1 than hMCL-SCs (A). In addition, qRT-PCR showed that hACL-SCs expressed more than 300-fold lower levels of the STRO-1 gene than hMCL-SCs (B). Similarly, fewer hACL-SCs expressed Oct-4 than hMCL-SCs (C), and Oct-4 gene expression by hACL-SCs was more than 7 times lower than that of hMCL-SCs (D). Note that the results shown here were obtained from a 20-year-old male donor (see Table 1). The passage 1 cells were used in immunostaining. (Bar: 100 μm).