Histochemical staining of differentiated cells and semi-quantification of the extent of cell differentiation. Both hACL-SCs and hMCL-SCs were able to differentiate into adipocytes (A), chondrocytes (C), and osteocytes (E), as shown by the accumulation of lipid droplets, proteoglycans, and calcium deposits on cell surfaces. However, the extent of hACL-SC differentiation was less than that of hMCL-SC differentiation, evidenced by smaller positive staining areas for adipogenesis (B), chondrogenesis (D), and osteogenesis (F) in hACL-SCs than in hMCL-SCs. Note that each experiment was repeated three times using three different donors. (Bar: 100 μm).