Figure 6

Apoptosis, active-caspase expression, angiogenesis and dilated lymphatic vessels with cancer cell invasion in mammary carcinomas. TUNEL-positive apoptotic cells are much more frequently seen in the tumor of a mouse given 20 mg/kg/day α-mangostin (B) than in the tumor of a control mouse (A). Expression of active caspase-3 (C, D) and active caspase-9 (E, F) was more prominent in the tumor of a mouse given 20 mg/kg/day α-mangostin (D, F) than in a control mouse (C, E). The number of CD-31-positive endothelial cells was lower in the tumor of a mouse given 20 mg/kg/day α-mangostin (H) compared to that of a control mouse (G). Podoplanin-positive lymphatic vessels of a tumor in a control mouse were often dilated and filled with tumor cells (arrow, I). Mammary cancer cells were also observed in the intraluminal space of the dilated lymphatic vessels in the tumor of a mouse given 20 mg/kg/day α-mangostin (arrow, J). (A-H): Scale bar = 50 μm; (I, J): Scale bar = 25 μm. (A, B): TUNEL stain; (C, D): active caspase-3 immunohistochemistry; (E, F): active caspase-9 immunohistochemistry; (G, H): CD31 immunohistochemistry; (I, J): podoplanin immunohistochemistry.