Fig. 2

AluDia translocation and biopersistence in injected muscle after 21 days. a: immunohistochemistry analysis: AluDia particle accumulation in the injected muscle inducing a granuloma of CD11b+ monocyte-macrophage lineage cells filled with AluDia in the endomysium area. The green fluorescence corresponds to CD11b protein immunolabeling. b-d: AluDia particle detection (b) in injected muscle non-stained with Morin. Tissue section was observed using the 420 nm excitation for Morin (c) and phase contrast (d). e-g: the stable association between the mfND component as assessed by the red fluorescence obtained with the 532 nm laser excitation (e) and alum after Morin staining observed with 420 nm excitation (f) and phase contrast image (g). h: mfND component of AluDia complex (red fluorescence) with alum component stained with Morin and disturbed by the high fluorescent background of muscle cells (i) at the injection site in muscle section observed with phase contrast microscopy analysis (j). AluDia Alhydrogel^® and mfND complex, mfNDs modified fluorescent nanodiamonds