Fig. 3

pSTAT3 and pSTAT3-GS are associated with PTEN loss and stromal reactivation. a pSTAT3 is associated with PTEN loss. Heatmap representation of the correlations between the RPPA values in the Responsify dataset. Cells are colored according to Pearson correlation coefficient values, with green indicating positive correlation and red negative correlations. b pSTAT3-GS is associated with stromal reactivation. The heatmap reflects the hierarchic clustering of pairwise correlations between different gene signatures in the Responsify dataset. Cells are colored according to Pearson correlation coefficient values, with green indicating positive correlation and red negative correlations. c The heatmap of the top significantly enriched genes in PAM50-identified patients with HER2-positive breast cancer annotated in the TCGA (P <0.001, fold >1), selected according to the high or low RPPA expression level of pSTAT3. Cells are colored according to the gene expression values, with green indicating positive correlation and red negative correlation. d pSTAT3-GS correlates with histological stromal reactivation. Histological sections showing breast tumors containing low (+), intermediate (++), and high (+++) reactive stroma. Heatmap shows correlation of reactive stromal content with clinical pathological parameters (not significant) and correlation with different gene signatures including stromal signature and pSTAT3-GS. * Negative correlation, P values were assessed using Mann–Whitney test