Fig. 1

Low PARP1 protein expression in ovarian tumors. a Analysis of The Cancer Genome Atlas (TCGA) ovarian cystadenocarcinoma database for PARP1 mRNA (n = 570) and protein (n = 412) expression levels. Data were used to generate a heat map with red representing high expression level and green low expression level. b Pie chart representing PARP1 protein level as determined by immunohistochemistry (IHC) in 69 HGSC samples (training cohort). c PARP1 representative IHC for each expression category (low and high). d Pie chart distribution of PARP1 in 100 unselected ovarian tumors from 74 patients (validation cohort 2, several patients had multiple tumors examined) evaluated by western blotting. e PARP1 representative western blot for each expression category (low, high, and negative); OVCAR4 protein extract was used as positive control. f Pie chart representing BRCA1 protein level distribution from 74 patients (100 tumors, validation cohort 2) evaluated by western blotting