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Fig. 1 | BMC Medicine

Fig. 1

From: RNY-derived small RNAs as a signature of coronary artery disease

Fig. 1

Apoptotic and atherogenic stimuli induce s-RNY expression in macrophages. a MA plot distribution from the high throughput sequencing analysis of differentially expressed small RNAs in human primary macrophages stimulated with 1 μM of staurosporine (STS) for 6 hours compared to control. Green dots indicated RNYs. b Northern blot detecting the indicated s-RNYs in apoptotic mouse bone marrow-derived macrophages (BMDMs) upon 36 h of M-CSF withdrawal treatment or reconstitution after 12 h of starvation. U6 snRNA was used as loading control. CTL indicates control. c RT-qPCR analysis of the indicated s-RNYs in mouse BMDMs incubated for 28 h with 0.25 μM of thapsigargin (Tg) alone or in combination with the indicated concentrations of oxidized LDL. The data were normalized by U2 snRNA and presented as mean and standard deviation (n per group = 8). d RT-qPCR analysis of the indicated s-RNYs in mouse BMDMs incubated for 18 h with 0.25 μM of Tg with bovine serum albumin (BSA), 0.25 mM of the indicated fatty acids in complex with BSA, or Tg plus the fatty acids. The data were normalized by U2 snRNA and presented as mean and standard deviation (n per group = 8). The unsaturated fatty acids were linoleic acid (LA) and oleic acid (OA), and the saturated fatty acids were stearic acid (SA) and palmitic acid (PA). RT-qPCR analysis of the indicated s-RNYs in control and ApoE −/− (e) or Ldlr −/− (f) aortic arches. The mice were fed with either chow diet or high cholesterol diet. The data were normalized by U2 snRNA and presented as mean and standard deviation (n per group = 8 for (e) and n per group = 5 for (f)). Student’s t-test: *P <0.05; **P <0.01

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