Fig. 5
From: RNY-derived small RNAs as a signature of coronary artery disease
s-RNY1-5p is stable in extracellular environment. a Northern blot analysis showing the stability of s-RNY1-5p and U6 snRNA in the medium of bone marrow-derived macrophages stimulated with 1 μM of staurosporine at the indicated time points. Total RNA was isolated from the medium. b, c RT-qPCR analysis from three coronary artery disease patients showing the stability of s-RNY1-5p in the serum in either prolonged room temperature incubation (b) or different cycles of freeze-thaw as indicated (c). Data were normalized by using the synthetic non-mammalian cel-miR-39, which was added to the samples before the RNA extraction. Each ΔCt (corresponding to Cts-RNY1-5p-Ctcel-miR-39) is the average of three technical replicates