Fig. 6

Immunostaining analysis of renal (pro)renin receptor (PRR) expression during angiotensin II (AngII)-induced hypertension. a Validation of specificity of the labeling. (a) Immunostaining of PRR antibody in kidney; (b) Immunostaining in the kidney in the presence of the immune PRR peptide (Cat#ab41522, Abcam); (c) Immunostaining of the kidney with rabbit IgG protein only; (d) Immunostaining of PRR in the heart. b Co-labeling with anti-PRR antibody and anti-α-SMA antibody in control group (e–h) and AngII group (i–l). The kidney section was stained with anti-PRR antibody and anti-α-SMA antibody (e–g; i–k). The merged images are shown in (h) and (l). (e) and (i): 100× magnification; (f–h) and (j–i): 400× magnification. Shown are representatives of three to six animals per group