Fig. 2

Transcriptional changes before versus after differentiation of human primary myoblasts of non-obese subjects. a Significant changes in mRNA expression of key myogenic transcription factors in human myoblasts (n = 13) versus myotubes (n = 13) of non-obese subjects. Significantly enriched downregulated (b) and upregulated (c) gene sets in Gene Set Enrichment Analysis with FDR < 5% (q < 0.05) based on differential gene expression in human myoblasts versus myotubes of non-obese subjects. Significant changes in mRNA expression of genes encoding cell cycle proteins (d), myosin heavy and light chains (e), proteins in the respiratory chain (f), and cytokines and their receptors (g) in human myoblasts versus myotubes of non-obese subjects (n = 13). Data are presented as mean ± SEM. ECM, Extracellular matrix; Val, Valine; Leu, Leucine; Ile, Isoleucine. * q < 0.05