Table 1 Comparison of paired EMS-spot samples collected pre- and post-treatment
|  | Paired pre-treatment samples (n = 1115) |  | Paired post-treatment samples (n = 2995) | |||||||||
Smear microscopy | Â | Â | Spot | Â | Â | Â | Â | Spot | Â | Â | ||
Negative | Positive | Total | Negative | Positive | Total | |||||||
EMS | Negative | 1 (0.1%) | 33 (3.0%) | 34 (3.0%) | Â | EMS | Negative | 2853 (95.3%) | 15 (0.5%) | 2868 (95.8%) | Â | |
Positive | 17 (1.5%) | 1064 (95.4%) | 1081 (97.0%) | Positive | 24 (0.8%) | 103 (3.4%) | 127 (4.2%) | |||||
| Â | Total | 18 (1.6%) | 1097 (98.4%) | 1115 | Â | Â | Total | 2887 (96.4%) | 118 (3.9%) | 2995 | ||
|  |  |  |  | P = 0.02 |  |  |  |  |  | P = 0.15 |  | |
Smear grading | Â | Agreement (both positive) | 506 (47.6%) | Â | Â | Agreement (both positive) | 71 (68.9%) | Â | ||||
Grading higher on EMS | 409 (38.4%) | Â | Grading higher on EMS | 24 (23.3%) | Â | |||||||
Grading higher on spot | 149 (14.0%) | χ2 P < 0.001 | Grading higher on spot | 8 (7.8%) | χ2 P = 0.002 | |||||||
Total (where both positive) | 1064 | Â | Total (where both positive) | 103 | Â | |||||||
LJ culture | Â | Â | Spot | Â | Â | Â | Spot | Â | ||||
Negative | Positive | Contaminated | Total | Negative | Positive | Contaminated | Total | |||||
EMS | Negative | 18 (1.6%) | 48 (4.3%) | 10 (0.9%) | 76 (6.8%) | EMS | Negative | 2129 (71.1%) | 52 (1.7%) | 247 (8.2%) | 2428 (81.1%) | |
Positive | 30 (2.7%) | 826 (74.1%) | 61 (5.5%) | 917 (82.2%) | Positive | 31 (1.0%) | 85 (2.8%) | 11 (0.4%) | 127 (4.2%) | |||
Contaminated | 8 (0.7%) | 91 (8.2%) | 23 (2.1%) | 122 (10.9%) | Contaminated | 268 (8.9%) | 10 (0.3%) | 162 (5.4%) | 440 (14.7%) | |||
| Â | Total | 56 (5.0%) | 965 (86.5%) | 94 (8.4%) | 1115 | Â | Total | 2428 (81.1%) | 147 (4.9%) | 420 (14.0%) | 2995 | |
|  |  |  |  |  | P = 0.006 |  |  |  |  |  | P = 0.11 | |
MGIT culture | Â | Â | Spot | Â | Â | Â | Spot | Â | ||||
Negative | Positive | Contaminated/false positive | Total | Negative | Positive | Contaminated/false positive | Total | |||||
EMS | Negative | 2 (0.2%) | 12 (1.1%) | 1 (0.1%) | 15 (1.3%) | EMS | Negative | 1979 (66.1%) | 67 (2.2%) | 227 (7.6%) | 2273 (75.9%) | |
Positive | 9 (0.8%) | 998 (89.5%) | 34 (3.0%) | 1041 (93.4%) | Positive | 73 (2.4%) | 119 (4.0%) | 36 (1.2%) | 228 (7.6%) | |||
Contaminated/false positive | 3 (0.3%) | 49 (4.4%) | 7 (0.6%) | 59 (5.5%) | Contaminated/false positive | 288 (9.6%) | 46 (1.5%) | 160 (5.3%) | 494 (16.5%) | |||
| Â | Total | 14 (1.3%) | 1059 (95.0%) | 42 (3.8%) | 1115 | Â | Total | 2340 (78.1%) | 232 (7.7%) | 423 (14.1%) | 2995 | |
|  |  |  |  |  | P = 0.18 |  |  |  |  |  | P = 0.01 | |