Skip to main content
Fig. 2 | BMC Medicine

Fig. 2

From: Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury

Fig. 2

CRAMP reduces cardiomyocyte apoptosis via activating Akt and ERK1/2. a, b Western blot analysis for Akt and ERK1/2 phosphorylation after treatment of neonatal rat cardiomyocytes (NRCMs) with the rCRAMP peptide (a, n = 6) or the siRNA targeting rCRAMP (b, n = 6), in the presence or absence of oxygen glucose deprivation/reperfusion (OGDR) treatment. All membranes were probed, stripped, and then reprobed for determining the phosphorylation levels of Akt and ERK1/2. cf The apoptosis of OGDR-treated NRCMs after treatment with the Akt inhibitor MK2206 or the MEK inhibitor PD98059 together with the rCRAMP peptide as determined by TUNEL staining (c, e, n = 4) and Western blot (d, f, n = 6). Immunofluorescent staining for α-actinin was used to label cardiomyocytes. Scale bar = 100 μm (c, e). Data were expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page