Fig. 4

CRAMP reduces cardiac I/R injury in vivo. a, b Mice were intraperitoneally injected with the mCRAMP peptide, and the infarct size and myocardial apoptosis after I/R injury were analyzed by 2,3,5-triphenyltetrazolium chloride (TTC) staining (a, n = 11) and TUNEL staining (b, n = 4). Immunofluorescent staining for α-actinin was used to label cardiomyocytes. c, d The ratio of Bax/Bcl-2 and Caspase-3 cleavage, in addition to the Akt, ERK1/2, and FoxO3a phosphorylation levels, were analyzed by Western blot after mCRAMP treatment in both the infarct (c) and border (d) zones of mice I/R hearts (n = 3). All membranes were probed, stripped, and then reprobed for determining the phosphorylation levels of Akt, ERK1/2, and FoxO3a. Scale bar = 20 μm (b). Data were expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001