Fig. 3
From: Synthetic high-density lipoprotein nanoparticles for the treatment of Niemann–Pick diseases
sHDLs modulate cholesterol regulatory genes. a Primary fibroblasts homozygous for NPC1 I1061T were treated with vehicle (Veh), cyclodextrin (cyclo), or 5A-SM at the indicated concentrations for 48 h. HMGCR, HMGCS1, ABCA1, LDLR, SREBP, or NPC1 mRNA expression was analyzed by qPCR. b, c NPC1 protein in control (CTRL) and NPC1 I1061T (NPC) fibroblasts was analyzed by b western blot (quantified at right) or c digested with endoglycosidase H (E), PNGase F (P), or not treated (NT) and analyzed by western blot. Data are mean ± s.e.m. from three independent experiments. n.s., not significant, *p ≤ .05, **p ≤ .01, ***p ≤ .001, ****p ≤ .0001 relative to Veh by a one-way ANOVA with Tukey post hoc test (F, df = (HMGCR) 27.43, df = 5; (HMGCS1) 24.75, 5; (ABCA1) 43.54, 5; (LDLR) 9.4, 5; (SREBP) 7.0, 5; (NPC1) 12.41, 4. b Student’s t test t = 3.83, df = 2