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Fig. 4 | BMC Medicine

Fig. 4

From: NG2 glia regulate brain innate immunity via TGF-β2/TGFBR2 axis

Fig. 4

Ablation of NG2 glia results in hyper-responsiveness of microglia to LPS stimulation. a Immunofluorescent histochemical staining for IBA1 in the cerebrocortex of LPS-treated DTRNG2 Tg and control mice. Scale bars, 50 μm. b Quantitative estimation of IBA1-positive microglia in the data shown in a. ce Quantitative assessment of IBA1-positive microglia diameters (c), endpoints/cell (d), and process length/cell (e) in mice with ablated NG2 glia and/or challenged with LPS. Two-way ANOVA with Tukey’s multiple comparisons test, (n = 3–5 mice), *P < 0.05. f A heatmap of microglia-enriched genes (|log2(fold change)| > 1 and Padj < 0.05 cutoff) regulated by ablation of NG2 glia. Profiles are shown in a color scale where blue is low and red is high. g Read counts of microglia-specific genes that show a twofold decrease in DTRNG2 saline mice compared to CTRL saline mice. Cx3cr1, P2ry13, P2ry12, and Csf1r also show a twofold decrease in DTRNG2 LPS mice compared to CTRL LPS mice. h The expression of CX3CR1 is maintained in a NG2 glia-dependent manner in adult mouse brain in vivo. qPCR analysis of the indicated brain regions, including the cerebrocortex and hippocampus of adult mice with ablated NG2 glia 4 h after systemic LPS (2.5 mg kg−1, i.p.) administration. Two-way ANOVA with Bonferroni’s post test (n = 5–6 mice per group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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