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Fig. 5 | BMC Medicine

Fig. 5

From: RETRACTED ARTICLE: Therapeutic effect of nanoliposomal PCSK9 vaccine in a mouse model of atherosclerosis

Fig. 5

Expression (a, b) and cellular distribution (c, d) of LDLR in the livers of immunized hypercholesterolemic mice. Liver extracts were separated by SDS-PAGE and blotted onto a nitro-cellulose filter. Following incubation with HRP-conjugated anti-LDLR or anti-β actin antibody, the bands corresponding to LDLR or β-actin proteins were measured by ImageJ software. The LDLR bands were normalized on the corresponding β-actin band (a). Compared to the control group, the protein expression of LDLR in the liver was significantly increased in the vaccine group. Data are expressed as means ± SEM (n = 7 per group). Differences at p values less than 0.05 were considered to be statistically significant (b). The cellular distribution of LDLR in immunized mice (c, d). Immunohistochemistry analysis was carried out on the liver samples isolated from the control (c) or vaccinated (d) mice using rabbit anti-LDLR antibodies detected by anti-rabbit IgG HRP-conjugated antibodies

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