Skip to main content
Fig. 1 | BMC Medicine

Fig. 1

From: Mass cytometry analysis reveals a distinct immune environment in peritoneal fluid in endometriosis: a characterisation study

Fig. 1

Peritoneal fluid cells show complex phenotypic heterogeneity. a viSNE plots showing the composite profiles of PFCs and PBCs. Haematopoietic cells from all PF (n = 20) and all blood (n = 20) samples were used for the analysis. Clouds of cells are generated by viSNE analysis. Each dot in the plots represents a single cell, and its colour suggests its immune cell type derived from manual gating (see Additional file 1: Table S3 and Additional file 1: Figure S2). b Phenotypic mapping of PFCs shown by minimum spanning tree plot. A composite plot of all PF samples is shown (plots from each sample are listed in Additional file 2). Each node represents a cell cluster, and node size indicates abundance of the cluster. X-shift algorithm identified 44 subpopulations (k = 40) that are named according to their ranking of proportions in all PFCs (from group 1 to group 44). Percentage in total cells of each group from group 1 to group 11 are labelled. Proportions of all other groups (group 12 to group 44) are below 1%. c Expression phenotypes of markers in these clusters are shown in the heat map (each row represents an individual cluster; numbers on the left indicate group names; black represents the minimum, yellow represents the median, and red represents the maximum expression value). These subpopulations were hierarchically clustered based on their marker expression patterns. d Spanning tree plots showing expression of activation markers on macrophage clusters. M1 and M2 activation markers are co-expressed on macrophages. A marker with negative expression (CD20) is also shown. Colour scales indicate intensities of markers. Group IDs are labelled in the plots

Back to article page