Fig. 3

CD69 expression on T cells is increased in endometriosis PF. Abundances of group 5 in CD8 T cells (a) and combination of three groups, 11, 19, and 41, in CD4 T cells (b) are increased in endometriosis PF samples (n = 14) compared to controls (n = 6). Endo, endometriosis. c Stacked plots showing expression of T cell early activation markers, CD69, on T cells using manual gating suggest that CD69⁺ T cells are increased specifically in PF samples from endometriosis patients. d, e CD69 expression profiles in PF and blood samples. The spanning tree plots show hierarchies of all cell clusters in PF (d) and blood (e) samples generated from X-shift analysis. Each node represents a cell cluster, and node size indicates abundance of the cluster. Colour scales indicate intensities of CD69, suggesting that it is predominantly expressed on T cells in PFCs. Groups 5, 11, 19, and 41 from PFCs are labelled on tree plot. Means ± SEM are shown in scatter plots. The Mann-Whitney U test was used in all statistical calculations. *p < 0.05