Fig. 6
Similar to Drosophila, dystroglycan (DAG1) and Kibra interact in mammalian muscles. a Co-immunoprecipitation of beta-Dg (DAG1) and Kbr from mouse muscle extracts (tibialis anterior muscles, consisting of a mixture of oxidative and glycolytic muscle fibers) shows that the two proteins can form a complex in mammalian muscles. b Co-staining of mouse myotubes differentiated from C2C12 myocytes with anti-DAG1 (red) and anti-Kbr (green) antibodies. Arrowheads show the co-localization of both proteins at the sarcolemma of differentiated muscle cells in vitro. c Co-staining of the cryo-sections of mouse leg muscles (quadriceps femoris, a mixed slow- and fast-twitch oxido-glycolytic muscle) with anti-DAG1 and anti-Kbr antibodies demonstrate the co-localization of the proteins at the muscle sarcolemma in vivo, which is similar to our findings in Drosophila (see Additional file 2: Figure S2)